Macerating activity of endo-polygalacturonase produced by Saccharomyces fragilis.
نویسندگان
چکیده
منابع مشابه
Isolation and Characterization of Polygalacturonase Produced by Tetracoccosporium sp.
Thirty-five fungal strains which isolated from vegetable wastes, were screened for the use of polygalacturonic acid as the sole carbon source. Twenty-five isolates were positive for polygalacturonase activity in cup-plate assay, as evidenced by clear hydrolysation zones. The most productive strain was determined by measuring clear zones formed around colonies stained with ruthenium red.</em...
متن کاملisolation and characterization of polygalacturonase produced by tetracoccosporium sp.
thirty-five fungal strains which isolated from vegetable wastes, were screened for the use of polygalacturonic acid as the sole carbon source. twenty-five isolates were positive for polygalacturonase activity in cup-plate assay, as evidenced by clear hydrolysation zones. the most productive strain was determined by measuring clear zones formed around colonies stained with ruthenium red. the hig...
متن کاملIdentification of endo- and exo-polygalacturonase activity in Lygus hesperus (Knight) salivary glands.
Polygalacturonase (PG) activity found in the salivary gland apparatus of the western tarnished plant bug (WTPB, Lygus hesperus Knight) has been thought to be the main chemical cause of the damage inflicted by this mirid when feeding on its plant hosts. Early viscosity and thermal stability studies of the PG activity in L. hesperus protein extracts were difficult to interpret. Thus, it has been ...
متن کاملComprehensive glycan analysis of recombinant Aspergillus niger endo-polygalacturonase C.
The enzyme PGC is produced by the fungus Aspergillus niger during invasion of plant cell walls. The enzyme has been homologously overexpressed to provide sufficient quantities of purified enzyme for biological studies. We have characterized this enzyme in terms of its posttranslational modifications (PTMs) and found it to be both N- and O-glycosylated. The glycosyl moieties have also been chara...
متن کاملSeparation of endo-polygalacturonase using aqueous two-phase partitioning.
The partitioning of endo-polygalacturonase (endo-PG) in polyethylene glycol (PEG)-polyvinyl alcohol (PVA10000) and PEG-hydroxypropyl starch (Reppal PES100) aqueous two-phase systems was studied, and revealed the possibility of using aqueous two-phase extraction to purify and concentrate endo-PG from its clarified fermentation broth. For the PEG8000-PVA10000 system, endo-PG presented in the ferm...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1979
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.43.1369